High Yield of DNA and RNA in Nucleic Acid Extraction Using Reducing Agents

Abstract

Genetic extraction constitutes a pivotal step in biomolecular science. The utilization of reducing agents, such as β-mercaptoethanol, dithiothreitol, tris-2-carboxyethyl phosphine hydrochloride, and dithiobutylamine, in the extraction of genetic material from medical specimens has been shown to result in higher purity and concentration of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The objective of this study is to investigate the properties and efficacy of various reducing agents that can be utilized to enhance the process of genetic extraction. Reducing agents play a vital role in the process of disrupting disulfide bonds within the protein structures that encapsulate nucleic acids, thereby facilitating the efficient release of DNA and RNA. In addition, reducing agents have been shown to inhibit the activity of enzymes responsible for the breakdown of nucleic acids. At present, reducing agents are frequently utilized in automated nucleic acid extraction processes in conjunction with magnetic nanoparticles, thereby yielding highly concentrated DNA and RNA. Furthermore, it has been demonstrated to exhibit reduced extraction times. In summary, the development and application of reducing agents in the genetic material extraction process will improve the quality and efficiency of nucleic acid extraction, genetic analysis, and medical disease diagnosis.